Peasy®-blunt e1 expression vector
WebpEASY ®-Blunt E1 Expression Vector is constructed from pET vector, it utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient … WebApr 15, 2024 · The E. coli expression vector pEASY-Blunt E1-BvGSTU9 (TransGen) was generated by amplifying BvGSTU9 using the primers BvGSTU9-F (5’ …
Peasy®-blunt e1 expression vector
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WebThen msv was cloned into the prokaryotic expression vector pEASY -Blunt E1. The fusion protein msv was expressed by pEASY -Blunt E1 under the induction of isopropyl-β-d … WebNov 19, 2016 · The E. coli strains used were DH5α for DNA manipulation and BL21 (DE3) for protein expression. The pEASY-Blunt E1 plasmid (TransGen Biotech, China) was used as …
WebMay 1, 2024 · Expression analysis of the BoaZDS gene in E. coli The BoaZDS fragment was inserted into the pEASY-Blunt E1 vector, and a recombinant prokaryotic expression vector was constructed. The recombinant plasmid pEASY -Blunt E1- BoaZDS was then transformed into E. coli Transetta (DE3) cells. WebMay 26, 2024 · Using the TIANprep miniature plasmid kit (Tiangen, Beijing, China), the plasmid DNA were isolated from the recombinant clone, and sequencing confirmed the insert pEASY-Blunt no.e1-YtnP. Next, the recombinant plasmid pEASY Blunt E1-YtnP into E. coli BL21 (DE3) cells, 37 °C in containing 50 mg/mL of ampicillin LB culture medium for …
WebpEASY-Blunt Simple (linearized) Cloning vector for blunt-ended PCR products, with blue-white selection, kanamycin and ampicillin resistance markers, and a T7 promoter but no … WebOct 15, 2024 · Expression of boaugt74b1 in escherichia coli. UGT74B1 CDS was ligated with the pEASY-Blunt E1 expression vector and transformed into Trans1-T1 (TransGene, …
WebJun 2, 2024 · Briefly, the PCR products of target genes (without signal peptides) were purified and then cloned into a pEASY-Blunt E1 expression vector (TransGen). E. coli BL21 (DE3) cells containing the expression vector were grown at 37 °C to an OD600 of ~0.8 and induced with isopropyl-β-D-thiogalactopyranoside (IPTG) at a final concentration of 1 mM …
WebAug 26, 2024 · Briefly, the recombinant vector (pEASY-Blunt E1-PiGSTd1) was transformed into E. coli BL21 (DE3), and the positive clones were isolated for expression. Cultures … broderick carterWeb酿酒酵母(S.cerevisiaeCCIC 1002)由本实验室保藏；宿主菌EscherichiacoliBL21(DE3)为实验室已有资源；E.coliTrans-T1克隆宿主菌和载体pEASY-Blunt E1购自北京全式金(TransGen Biotech)生物技术有限公司. broderick building servicesWebFree Trial pEASY-Blunt E1 (linearized) Prokaryotic expression vector for cloning blunt-ended PCR products and adding an N-terminal 6xHis tag. Sequence Author: TransBionovo … broderick burger walnut creekbroderick californiaWebProduct Details. pEASY® -Blunt Cloning Kit is designed for cloning and sequencing Pfu-amplified PCR products. •5 minutes fast ligation of Pfu-amplified PCR products. … broderick car crashWebFeb 15, 2024 · 表3 表达载体构建体系Tab.3 Expression vector construction system 1.5 杜梨子叶浸染及遗传转化 杜梨子叶再生参照林静[18]的方法，遗传转化参照庞宏光[19]的方法，主要操作包括：杜梨种子消毒、子叶诱导愈伤培养、农杆菌介导的遗传转化、筛选培养基培 … broderick castleWebpEASY® - Blunt3 Cloning Vector provides dual EcoR I and dual Not I enzyme digestion sites. It is designed for cloning and sequencing Pfu-amplified PCR products. The cloned insert can be released from a single enzyme digestion. •5 minutes fast ligation of Pfu-amplified PCR products. •Ampicillin resistance gene for selection. broderick byas